Feasibility as a Laundry Detergent Additive of an Alkaline Protease from Bacillus clausii C5 Transformed by Chromosomal Integration
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چکیده
منابع مشابه
Optimization of alkaline protease production from a locally isolated Bacillus sp. ZR-5: Potential application as a detergent additive
The aim of this study was to optimize protease production using a novel Bacillus sp. ZR-5 strainisolated from the soil, and evaluate its application in detergent industry. The proteolytic activityof the strain was demonstrated using gelatin hydrolysis screening test. Protease productionoptimization was carried out using a two-step approach: a conventional method in order toidentify the best car...
متن کاملCloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis
in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کاملPurification and characterization of laundry detergent compatible alkaline protease from Bacillus cereus
An alkalophilic bacterium, Bacillus cereus produced an extracellular alkaline protease, which was found to be active at high temperature and pH range, suitable for commercial laundry detergents. B. cereus protease was partitioned in different aqueous two-phase systems such as PEG/dextran, PEG/potassium phosphate, PEG/sodium citrate, PEG/magnesium sulphate and PEG/sodium dihydrogen phosphate and...
متن کاملoptimization of alkaline protease production from a locally isolated bacillus sp. zr-5: potential application as a detergent additive
the aim of this study was to optimize protease production using a novel bacillus sp. zr-5 strainisolated from the soil, and evaluate its application in detergent industry. the proteolytic activityof the strain was demonstrated using gelatin hydrolysis screening test. protease productionoptimization was carried out using a two-step approach: a conventional method in order toidentify the best car...
متن کاملAn Alkaline Protease from Bacillus pumilus MP 27: Functional Analysis of Its Binding Model toward Its Applications As Detergent Additive
A proteolytic strain of Bacillus pumilus MP 27 was isolated from water samples of Southern ocean produced alkaline protease. Since protease production need expensive ingredients, an economically viable process was developed by using low cost carbon source, wheat straw, supplemented with peptone. This protease was active within temperature ranges 10-70°C at pH 9. This process was optimized by re...
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ژورنال
عنوان ژورنال: KSBB Journal
سال: 2012
ISSN: 1225-7117
DOI: 10.7841/ksbbj.2012.27.6.352